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COLOR PLATE
Color Plate 5.11 In situ detection of germ cell apoptosis in monkeys before (a) and after heat treatment (b). Cellular localization of apoptosis was characterized by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay. Methyl green was used as a counterstain. Many apoptotic germ cells (dark brown in color) were noted in testicular biopsies obtained 3 days after the first heat treatment (b) but not from control animal (a). Bax immunoactivity in the testis before (c) and after heat treatment (d). A portion of seminiferous tubule from a normal adult monkey showing that Bax is localized in the cytoplasm of germ cells, Sertoli cells and a small amount in Leydig cells (c), and from a monkey 3 days after the first heat treatment (d) displaying intense immunoactivity of Bax in apoptotic germ cells (arrows). Scale bar 25 pm. From Lue et al., 2002, with permission
Color Plate 5.12 (a) and (b) Immunofluorescence of epididymal cell lines cultured for 1 week after approximately 30 passages on a glass slide with supplemented IMDM with 10% fetal calf serum and stained with PEB-like protein antibody (20pg/ml). (c) and (d) Immunofluorescence of the cell lines cultured for 1-2 weeks after approximately 30 passages on glass slides with supplemented IMDM with 10% fetal calf serum, stained with a cytokeratin antibody (68 pg/ml). Cytokeratin immunofluorescent staining was green and Hoechst 33342-stained nuclei were blue. (a) PCI; (b) DC1; (c) PCI; (d) DC1. Bar 10pm. From Araki et al., 2002, with permission