Rodak's Hematology: Clinical Principles and Applications, 5th Ed.


Rodak’s Hematology: Clinical Principles and Applications fifth edition is reorganized into 7 parts and 45 chapters for enhanced pedagogy. Chapter highlights and new content are described as follows:

Part I: Introduction to hematology

Chapters 1 to 5 Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 preview the science of clinical laboratory hematology and include laboratory safety, blood specimen collection, microscopy, and quality assurance. The quality assurance chapter was significantly updated to include enhanced sections on statistical significance; assay validation with applications of the Student’s t test, ANOVA, linear regression, and Bland-Altman difference plots; and assessment of diagnostic efficacy.

Part II: Blood cell production, structure, and function

Chapters 6 and 7 use photomicrographs and figures to describe general cellular structure and function and the morphologic and molecular details of hematopoiesis. Chapters 812, and 13 discuss erythropoiesis, leukopoiesis, and megakaryopoiesis using numerous photomicrographs demonstrating ultrastructure and microscopic morphology. Chapters 9 and 10 examine mature red blood cell metabolism, hemoglobin structure and function, and red blood cell senescence and destruction. Iron kinetics and laboratory assessment in Chapter 11 was substantially updated with new figures and updated coverage of systemic and cellular regulation of iron. Chapter 13 includes detailed descriptions of platelet adhesion, aggregation, and activation with updated figures.

Part III: Laboratory evaluation of blood cells

Chapter 14 describes manual procedures such as microscopy-based cell counts, hemoglobin and hematocrit determinations, and point-of-care technology. Chapter 15 has been substantially updated to include descriptions and figures of the latest automated hematology analyzers. Chapter 16 describes peripheral blood film examination and the differential count correlation to the complete blood count. New figures correlate red blood cell and platelet histograms to their morphology. Chapter 17 follows up with bone marrow aspirate and biopsy collection, preparation, examination, and reporting. Chapter 18 describes methods for analyzing normal and pathologic cells of cerebrospinal fluid, joint fluid, transudates, and exudates, illustrated with many excellent photomicrographs.

Part IV: Erythrocyte disorders

Chapter 19 provides an overview of anemia and describes cost-effective approaches that integrate patient history, physical examination, and symptoms with the hemoglobin, red blood cell indices, reticulocyte count, and abnormal red blood cell morphology. Chapters 20 to 22 Chapter 20 Chapter 21 Chapter 22 describe disorders of iron and DNA metabolism and bone marrow failure. New algorithms help the reader to distinguish types of microcytic and macrocytic anemias. Chapters 23 to 26 Chapter 23 Chapter 24 Chapter 25 Chapter 26 discuss hemolytic anemias due to intrinsic or extrinsic defects. Chapter 23 is fully updated with new figures that detail extravascular and intravascular hemolysis and hemoglobin catabolism. Chapters 27 and 28 provide updates in pathophysiology, diagnosis, and treatment of hemoglobinopathies (such as sickle cell disease) and the thalassemias.

Part V: Leukocyte disorders

Chapter 29 is significantly updated with many excellent photomicrographs and summary boxes of nonmalignant systemic disorders manifested by the abnormal distribution or morphology of leukocytes. These include bacterial and viral infections, various systemic disorders, and benign lymphoproliferative disorders. Chapter 30 provides details on traditional cytogenetic procedures for detection of quantitative and qualitative chromosome abnormalities and more sensitive methods such as FISH and genomic hybridization arrays. Chapter 31 covers molecular diagnostics and was fully updated with new and enhanced figures on basic molecular biology, end-point and real-time polymerase chain reaction, microarrays, and DNA sequencing, including next generation sequencing. Chapter 32 describes flow cytometry and its diagnostic applications. It includes numerous scatterplots of normal and leukemic conditions. Chapters 33 to 36, with significant updating, provide the latest classifications and pathophysiologic models for myeloproliferative neoplasms, myelodysplastic syndromes, acute lymphoblastic and myeloid leukemias, chronic lymphocytic leukemia, and solid tumor lymphoid neoplasms, such as lymphoma and myeloma, with numerous full-color photomicrographs and illustrations.

Part VI: Hemostasis and thrombosis

Chapter 37 provides the plasma-based and cell-based coagulation models and the interactions between primary and secondary hemostasis and fibrinolysis with updated illustrations. Chapter 38 details hemorrhagic disorders, including the management of the acute coagulopathy of trauma and shock. Chapter 39 updates the currently recognized risk factors of thrombosis and describes laboratory tests that identify venous and arterial thrombotic diseases, particularly for lupus anticoagulant and heparin-induced thrombocytopenia (HIT) testing. Chapters 40 and 41 detail the quantitative and qualitative platelet disorders using additional tables and figures, and Chapter 42 details laboratory assays of platelets and the coagulation mechanisms with helpful new figures and diagrams. Chapter 43 covers the mechanisms and monitoring methods of the traditional warfarin and heparin-derived antithrombotic drugs, as well as all thrombin and factor Xa inhibitor drugs. It also includes methods for monitoring the different classes of antiplatelet drugs, including aspirin. Chapter 44 reviews the latest coagulation analyzers and point of care instrumentation.

Part VII: Hematology and hemostasis in selected populations

Chapter 45 provides valuable information on the hematology and hemostasis laboratory findings in the pediatric and geriatric populations correlated with information from previous chapters.


Rodak’s Hematology: Clinical Principles and Applications is designed for medical laboratory scientists, medical laboratory technicians, and the faculty of undergraduate and graduate educational programs in the clinical laboratory sciences. This text is also a helpful study guide for pathology and hematology-oncology residents and fellows and a valuable shelf reference for hematologists, pathologists, and hematology and hemostasis laboratory managers.

Textbook features

Elaine M. Keohane, PhD, MLS, Professor, Rutgers University, School of Health Related Professions, Department of Clinical Laboratory Sciences, co-editor in the fourth edition, and lead editor in the fifth edition, is joined by Larry J. Smith, PhD, Coagulation and Satellite Laboratory Director, Memorial Sloan Kettering Cancer Center, Adjunct Professor at Rutgers University, School of Health Related Professions and York College, CUNY, Department of Health Professions, and Jeanine M. Walenga, PhD, MT(ASCP), Professor, Loyola University Chicago, Stritch School of Medicine, Clinical Coagulation Laboratories Director, Loyola University Health System.

The outstanding value and quality of Rodak’s Hematology: Clinical Principles and Applications reflect the educational and clinical expertise of its current and previous authors and editors. The text is enhanced by nearly 700 full-color digital photomicrographs, figures, and line art. Detailed text boxes and tables clearly summarize important information. Reference intervals are provided on the inside front and back covers for quick lookup.

Each chapter contains the following pedagogical features:

• Learning objectives at all taxonomy levels in the cognitive domain.

• One or two case studies with open-ended discussion questions at the beginning of the chapter that stimulate interest and provide opportunities for application of chapter content in real-life scenarios.

• A bulleted summary at the end of each chapter that provides a comprehensive review of essential material.

• Review questions at the end of each chapter that correlate to chapter objectives and are in the multiple-choice format used by certification examinations.

• Answers to case studies and review questions that are provided in the Appendix.

The Evolve website has multiple features for the instructor:

• An ExamView test bank contains multiple-choice questions with rationales and cognitive levels.

• Instructor’s manuals for every chapter contain key terms, objectives, outlines, and study questions.

• Learning Objectives with taxonomy levels are provided to supplement lesson plans.

• Case studies have been updated and feature discussion questions and photomicrographs when applicable.

• PowerPoint presentations for every chapter can be used “as is” or as a template to prepare lectures.

• The Image Collection provides electronic files of all the chapter figures that can be downloaded into PowerPoint presentations.

For the student, a Glossary is available as a quick reference to look up unfamiliar terms electronically.